토토 후기학과 특별세미나 개최 안내
토토 후기학과에서는 특별세미나를 아래와 같이 개최하오니 관심있는 분들의 많은 참석을 바랍니다.
1. 연사: 강준호 박사 (KIST)
2. 일시: 2022. 11. 14(월), 오후 4시 30분
3. 장소: 공학3동 302호 (세미나실)
4. 제목: Physical dynamics of single 토토 후기s
Physical properties of 토토 후기s, such as mass, volume, and stiffness are valuable indicators of 토토 후기 state and function. Numerous 토토 후기ular processes including 토토 후기 development, differentiation, migration, and disease progression (e.g., cancer metastasis) accompany changes in 토토 후기ular physical properties. However, studying the physical dynamics is challenging because both the magnitude and duration of 토토 후기ular changes are heterogeneous across the 토토 후기 population. In this talk, we present new methods to monitor single-토토 후기 stiffness, bioenergetics, and mass over the entire 토토 후기 cycle with a temporal resolution of ~1 min.
We utilize the suspended microchannel resonator (SMR), a fluid-filled cantilever capable of measuring the buoyant mass of a 토토 후기 by the change of SMR resonant frequency. First, we quantify 토토 후기 stiffness via acoustic scattering of waves from a 토토 후기 inside the SMR. Through simulations, experiments with hydrogels, and chemical perturbation of 토토 후기s, we show that our readout from acoustic scattering measures stiffness1. Second, we monitor the mitochondrial membrane potential of single mouse lymphocytic leukemia L1210 토토 후기s and model ATP synthesis dynamics using an electrical circuit model of mitochondria2. We found that mitochondrial ATP synthesis temporarily decreases by approximately 50% during early mitosis. Lastly, we monitored L1210 토토 후기 growth during normal proliferation and polyploidization to show that increasing 토토 후기 mass by over 100-fold does not limit maximal growth efficiency, indicating exponential growth3. Altogether, these studies revealed physical dynamics and their underlying mechanism throughout the 토토 후기 cycle that have not been observed by endpoint assays.
[1] Kang, et al. “Non-invasive monitoring of single-토토 후기 mechanics by acoustic scattering”. Nature Methods (2019).
[2] Kang*, Katsikis*, et al. “Monitoring and modeling of lymphocytic leukemia 토토 후기 bioenergetics reveals decreased ATP synthesis during 토토 후기 division”. Nature Communications (2020).
[3] Mu*, Kang*, et al. “Mass measurements during lymphocytic leukemia 토토 후기 polyploidization decouple 토토 후기 cycle-and 토토 후기 size-dependent growth”. Proceedings of the National Academy of Sciences (2020).
-2.gif)
Login
